SRP042292|GSM1399295 Link out


2.0 | WBcel235

We follow the standard BED specification with 0-based coordinates. Additionally, we appended extra column(s). For more information click here.

The columns represent:

first - chromosome name

second and third - start and end positions of the poly(A) site cluster, respectively

fourth - unique cluster ID, composed of the chromosome name, the representative poly(A) site of the cluster and the strand. Note that this ID format is inspired by UCSC's position format, which uses 1-based coordinates instead of the 0-based bed coordinates used in the second and third columns. Thus, to convert the position of the representative site to bed coordinates, subtract 1.

fifth - expression (tags per million, tpm) in this sample

sixth - strand on which the cluster is encoded

seventh - percentage of samples that support the particular cluster

eighth - number of different 3' end sequencing protocols that support the particular cluster

ninth - average expression (tags per million, tpm) across all samples

tenth - two letter code for the cluster annotation (in order of decreasing priority: TE, terminal exon; EX, exonic; IN, intronic; DS, 1,000 nt downstream of an annotated terminal exon; AE, anti-sense to an exon; AI, anti-sense to an intron; AU, 1,000 nt upstream in anti-sense direction of a transcription start site; IG, intergenic)

eleventh - information about the poly(A) signal(s) that are present upstream of the poly(A) site, including the motif, the location with respect to the cleavage site and the genomic coordinate

Download gzipped BED file Add as custom track @ UCSC genome browser

The data was originally published in: Elewa, A. et al. POS-1 Promotes Endo-mesoderm Development by Inhibiting the Cytoplasmic Polyadenylation of neg-1 mRNA. Dev Cell 34, 108–118 (2015). Link out